Geometric cue for protein localization in a bacterium.

SEIMCHA: a new semantic image CAPTCHA using geometric transformations

As protection of web applications are getting more and more important every day, CAPTCHAs are facing booming attention both by users and designers. Nowadays, it is well accepted that using visual concepts enhance security and usability of CAPTCHAs. There exist few major different ideas for designing image CAPTCHAs. Some methods apply a set of modifications such as rotations to the original imag...

A mechanism for polar protein localization in bacteria.

We investigate a mechanism for the polar localization of proteins in bacteria. We focus on the MinCD/DivIVA system regulating division site placement in the rod-shaped bacterium Bacillus subtilis. Our model relies on a combination of geometric effects and reaction-diffusion dynamics to direct proteins to both cell poles, where division is then blocked. We discuss similarities and differences wi...

Physical Mathematics Seminar Interfacial Phenomena in Bacteria

I will present two experimental studies that deal with interfacial processes in bacteria. The adhesion of individual bacterial cells to a surface is a complex process, which involves the cell membrane, but is also mediated by surface appendages such as pili and flagella. We have used a microfluidic approach to study the adhesion of Pseudomonas aeruginosa to abiotic surfaces as a function of she...

A New Paradigm in Biology – The Effect of Localization of a Protein on its Anti-genicity

Intracellular Localization of FLAG-Peroxisomal Protein in Chinese Hamster Ovary (CHO) Cells

Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a  chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...